Neuronal Analysis

Specialized tools for spike and burst detection, spike cutouts, network bursts, and automated dose-response curves.

Both Multiwell-Screen and Multiwell-Analyzer include powerful analysis tools tailored to neuronal data. These tools extract multiple measures from the data on a single channel level and for the full network to best characterize the cell activity. Dose-response curves are automatically calculated for all these measures and all analysis results may be exported to Excel-compatible text files, as well as a PDF report.

Algorithms

The integrated spike detector adapts to the noise level of the data and extracts the individual spike events for each channel together with a short cutout representing the spike waveform. Spike detection is possible based on thresholds for either the raw signal or the signal slope.

The timing of the individual spike events is used by the burst detector to detect and extract measures for neuronal bursting.

A network burst detector analyzes the bursting activity of all channels in a well to identify synchronized bursting activity throughout the culture. All of these detection algorithms are highly customizable for perfect adaptation to your data.

Plate Overviews

To get a feeling for the activity and behavior of the cultures in all wells on a plate during the experiment, the Multiwell-Screen software visualizes spiking, bursting and network bursting activity for the full plate, making it easy to spot interesting wells, e.g. with regular synchronous bursting.

Well plate overview with spikes or network bursts

Stimulation-triggered Analysis

When using electrical or optical stimulation, the analysis can be performed relative to the stimulation time points to average the stimulation-evoked neuronal responses. The peri-stimulus time histogram (PSTH) display shows these responses as raster plots and histograms.